Biology · Textbook solutions

Biotechnology: Principles and Processes

Every solved example, exercise, and miscellaneous question, in the order the textbook teaches them. · 17 questions

Exercises

17 q

Ex 9 Q1 $Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).$
Ex 9 Q2 $Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.$
Ex 9 Q3 $From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?$
Ex 9 Q5 $Do eukaryotic cells have restriction endonucleases? Justify your answer.$
Ex 9 Q6 $Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?$
Ex 9 Q7 $Collect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.$
Ex 9 Q8 $Can you recall meiosis and indicate at what stage a recombinant DNA is made?$
Ex 9 Q9 $Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?$
Describe briefly the following:
Ex 9 Q10 (a) $Origin of replication$
Ex 9 Q10 (b) $Bioreactors$
Ex 9 Q10 (c) $Downstream processing$
Explain briefly
Ex 9 Q11 (a) $PCR$
Ex 9 Q11 (b) $Restriction enzymes and DNA$
Ex 9 Q11 (c) $Chitinase$
Discuss with your teacher and find out how to distinguish between
Ex 9 Q12 (a) $Plasmid DNA and Chromosomal DNA$
Ex 9 Q12 (b) $RNA and DNA$
Ex 9 Q12 (c) $Exonuclease and Endonuclease$