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Biology · Textbook solutions

Biotechnology

Every solved example, exercise, and miscellaneous question, in the order the textbook teaches them. Plus every board question asked from this chapter. · 23 questions

Exercise

23 q

Q1. Choose the correct option

Practice · 6
  1. Choose the correct option
    Ex Q1(1)
    The bacterium which causes a plant disease called crown gall is ................

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  2. Ex Q1(2)
    The enzyme nuclease hydrolyses ............... of polynucleotide chain of DNA.

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  3. Ex Q1(3)
    In vitro amplification of DNA or RNA segment is known as ...............

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  4. Ex Q1(4)
    Which of the following is the correct recognition sequence of restriction enzyme Hin d III.

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  5. Ex Q1(5)
    Recombinant protein .................. is used to dissolve blood clots present in the body.

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  6. Ex Q1(6)
    Recognition sequence of restriction enzymes are generally ............... nucleotide long.

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Q2. Very short answer type questions

Practice · 5
  1. Very short answer type questions.
    Ex Q2(1)
    Name the vector which is used in protuction of human insulin through recombinent DNA technology.
  2. Ex Q2(2)
    Which cells from Langerhans of pancreas do produce a peptide hormone insulin?
  3. Ex Q2(3)
    Give the role of Ca++\text{Ca}^{++} ions in the transfer of recombinent vector into bacterial host cell.
  4. Ex Q2(4)
    Expand the following acronyms which are used in the field of protechnology. i. YAC ii. RE iii. dNTP iv. PCR v. GMO vi. MAC
  5. Ex Q2(5)
    Fill in the blanks and complete the chart.
    GMOPurpose
    i. Bt cotton....................
    ii. ..................Delay the softening of tomato during ripening.
    ii. Golden rice....................
    iv. Holstein cow....................

Q3. Short answer type questions

Practice · 4
  1. Short answer type questions.
    Ex Q3(1)
    Explain the properties of a good and ideal cloning vector for rDNA technology.
  2. Ex Q3(2)
    In a PCR machine the temperature can be raised upto 100 0C100\ ^{0}\text{C} but after that it is not able to lower the temperature below 70 0C70\ ^{0}\text{C} automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
  3. Ex Q3(3)
    In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
  4. Ex Q3(4)
    Match and write the pairs.
    Recombinant proteinIt’s use in or for
    i. platelet derived growth factora. Anemia
    ii. α\alpha-antitrypsinb. cystic fobrosis
    iii. Relaxinc. Haemophilia A
    iv. Erythropoietind. Diabetes
    v. Factor VIIIe. Emphysema
    vi. DNA asef. Parturition
    g. Atherosclerosis

Q4. Long answer type questions

Practice · 8
  1. Long answer type questions.
    Ex Q4(1)
    Define and explain terms. i. Biopiracy ii. Biopatent iii. Bioethus
  2. Ex Q4(2)
    Explain the steps in process of rDNA technology with suitable diagrams.
  3. Ex Q4(3)
    Explain the gene therapy. Give two methods of the same.
  4. Ex Q4(4)
    How are the transgenic mice used in cancer research?
  5. Ex Q4(5)
    Give the steps in PCR or polymerase chain reaction with suitable diagrams.
  6. Ex Q4(6)
    What is a vaccine? Give advantages of oral vaccines or edible vaccines.
  7. Ex Q4(7)
    Enlist different types of restriction enzymes commonly used in rDNA technology? Write on their role.
  8. Ex Q4(8)
    Enlist and write in breif about the different biological tools required in rDNA technology.