Biotechnology and its Applications

112 CBSE Class 12 Biology past-year questions from 5 papers, 2022 to 2026, with answers and worked solutions.

Difficulty: 61 easy · 45 moderate · 6 hard.
Most-asked subtopics: Biotechnological Applications in Agriculture (55), Biotechnological Applications in Medicine (34), Transgenic Animals (15).
Updated 8 October 2026.

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#1Biotechnological Applications in Agriculture
#2Biotechnological Applications in Agriculture
#3Biotechnological Applications in Agriculture
#4Biotechnological Applications in Agriculture
#5Biotechnological Applications in Agriculture
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#6Biotechnological Applications in Agriculture
#7Biotechnological Applications in Agriculture
#8Biotechnological Applications in Agriculture
#9Biotechnological Applications in Agriculture
#10Biotechnological Applications in Agriculture
#11Biotechnological Applications in Agriculture
#12Biotechnological Applications in Agriculture
#13Biotechnological Applications in Agriculture
#14Biotechnological Applications in Agriculture
#15Biotechnological Applications in Agriculture
#16Biotechnological Applications in Agriculture
#17Biotechnological Applications in Agriculture
#18Biotechnological Applications in Agriculture
#19Biotechnological Applications in Agriculture
#20Biotechnological Applications in Agriculture
#21Biotechnological Applications in Agriculture
For Questions number 13 to 16, two statements are given — one labelled as Assertion (A) and the other labelled as Reason (R). Select the correct answer to these questions from the codes (A), (B), (C) and (D) as given below.
#22Biotechnological Applications in Agriculture
#23Biotechnological Applications in Agriculture
#24Biotechnological Applications in Agriculture
For Questions number 13 to 16, two statements are given – one labelled as Assertion (A) and the other labelled as Reason (R). Select the correct answer to these questions from the codes (A), (B), (C) and (D) as given below.
#25Biotechnological Applications in Agriculture
#26Biotechnological Applications in Agriculture
For Questions number 13 to 16, two statements are given – one labelled as Assertion (A) and the other labelled as Reason (R). Select the correct answer to these questions from the codes (A), (B), (C) and (D) as given below.
#27Biotechnological Applications in Agriculture
#28Biotechnological Applications in Agriculture
#29Biotechnological Applications in Agriculture
#30Biotechnological Applications in Agriculture
For Questions number 13 to 16, two statements are given – one labelled as Assertion (A) and the other labelled as Reason (R). Select the correct answer to these questions from the codes (A), (B), (C) and (D) as given below.
#31Biotechnological Applications in Agriculture
#32Biotechnological Applications in Agriculture
#33Biotechnological Applications in Agriculture
#34Biotechnological Applications in Agriculture
#35Biotechnological Applications in Agriculture
#36Biotechnological Applications in Agriculture
#37Biotechnological Applications in Agriculture
#38Biotechnological Applications in Agriculture
RNA interference (RNAi) holds great potential as a therapeutic agent for the treatment of human diseases and as biocontrol agents in controlling pests in the field of agriculture. An experiment was carried to study the use of 'RNAi' for the potential treatment of disorders of cholesterol metabolism. Some people possess genetic mutations with elevated levels of ApoB gene which predisposes them to coronary artery diseases. Lowering the amount of ApoB can reduce the number of lipoproteins and lower the blood cholesterol. Tracy Zimmerman and her colleagues used RNAi in 2006 to reduce the level of ApoB in non human primates Cynomolgus monkeys. One group of monkeys were given RNAi treatment (small interfering RNAs, SiRNAs) (doses 1 mg/kg SiRNAs), second group of monkeys were given RNAi treatment (doses 2.5 mg/kg SiRNAs) and third group of monkeys were injected with saline. The results of the study are depicted in the graph below :
#39Biotechnological Applications in Agriculture
There are two different farm lands, one where Bt-cotton crop was cultivated and the other where non Bt-cotton crop (indigenous) was cultivated. Farmers responsible for this experimental cultivation were free to use the farming practices of their choice. During the cultivation period, the data was collected with respect to the amount of pesticide used, water required for irrigation and at harvesting time, the crop productivity. Based on the data collected, a bar graph was plotted which is shown below.
#40Biotechnological Applications in Agriculture
There are two different farm lands, one where Bt-cotton crop was cultivated and the other where non Bt-cotton crop (indigenous) was cultivated. Farmers responsible for this experimental cultivation were free to use the farming practices of their choice. During the cultivation period, the data was collected with respect to the amount of pesticide used, water required for irrigation and at harvesting time, the crop productivity. Based on the data collected, a bar graph was plotted which is shown below.
#41Biotechnological Applications in Agriculture
There are two different farm lands, one where Bt-cotton crop was cultivated and the other where non Bt-cotton crop (indigenous) was cultivated. Farmers responsible for this experimental cultivation were free to use the farming practices of their choice. During the cultivation period, the data was collected with respect to the amount of pesticide used, water required for irrigation and at harvesting time, the crop productivity. Based on the data collected, a bar graph was plotted which is shown below.
#42Biotechnological Applications in Agriculture
#43Biotechnological Applications in Agriculture
#44Biotechnological Applications in Agriculture
#45Biotechnological Applications in Agriculture
#46Biotechnological Applications in Agriculture
#47Biotechnological Applications in Agriculture
RNA interference (RNAi) holds great potential as a therapeutic agent for the treatment of human diseases and as a bicontrol agent for controlling pests in the agricultural fields. An experiment was conducted to study the use of 'RNAi' for the potential treatment of disorders of cholesterol metabolism. Some people possess genetic mutations with elevated levels of 'ApoB' gene which predisposes them to coronary artery diseases. Lowering the amount of 'ApoB' can reduce the number of lipoproteins and lower the blood cholesterol. Tracy Zimmerman and her colleagues used RNAi in 2006 to reduce the level of 'ApoB' in non-human primates Cynomolgus monkeys. (i) One group of monkeys were given RNAi treatment (Small interfering RNAs, SiRNAs) (doses 1 mg/kg SiRNAs), (ii) Second group of monkeys were given RNAi treatment (doses $2{\cdot}5$ mg/kg SiRNAs), and (iii) Third group of monkeys were injected saline as control . The results of the experiments are illustrated in the graph given below :
#48Biotechnological Applications in Agriculture
#49Biotechnological Applications in Agriculture
#50Biotechnological Applications in Agriculture

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